18+ tm calculator neb
Web The NEB Tm Calculator is recommended to calculate an appropriate annealing temperature. When primers with annealing temperatures above 65C are used a 2-step.
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Web NEBs public Tm API allows programmatic access under our Terms of Service to the NEB Tm calculator for incorporation in automated high throughput primer design workflows.
. Web How to use the T m calculator. Web Please use NEBs Tm Calculator to determine the appropriate annealing temperature for your primer pair and polymerasebuffer of interest. The calculator calculates recommended T m melting temperature of primers and PCR annealing temperature based on the primer pair.
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